FIGURE

Fig. 5

ID

ZDB-FIG-220822-54

Publication

Issa et al., 2022 - A novel chemo-phenotypic method identifies mixtures of salpn, vitamin D3, and pesticides involved in the development of colorectal and pancreatic cancer

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Fig. 5

Fig. 5. (A) Protein-protein STRING Interaction map of nuclear receptors cross-talk with each other including VDR directly or indirectly (B). (C) Effect of salpn (10?�M, 16?h) in combination with D3 (orange) or D3 alone (blue) on transcription factor activity in HepG2 cells. HepG2 cells were transiently transfected with the optimized cis-FACTORIALTM library. Twenty-four hours after transfection cells were washed and supplied with fresh low serum (1% FBS, charcoal stripped) culture medium and treated with inducer for 16?h. Profile of the cis-FACTORIALTM activities was determined as fold of induction values versus vehicle-treated (DMSO) control cells. The graph shows fold-induction data plotted on a logarithmic scale. Insert table is a list of transcriptional pathways tested for their activity. (G) Wnt signaling modulation of salpn in zebrafish. (E) Wnt reporter activity was visualized in zebrafish using whole-mount in situ hybridization (WISH) for GFP expression.

Expression Data

Gene:	GFP
Fish:	w25Tg/w25Tg
Condition:	chemical treatment by environment: salpn
Anatomical Term:	head
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w25Tg/w25Tg
Condition:	chemical treatment by environment: salpn
Observed In:	head head regulation of canonical Wnt signaling pathway
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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