Fig. 5

Fig. 5 Fig. 5. (A) Protein-protein STRING Interaction map of nuclear receptors cross-talk with each other including VDR directly or indirectly (B). (C) Effect of salpn (10 µM, 16 h) in combination with D3 (orange) or D3 alone (blue) on transcription factor activity in HepG2 cells. HepG2 cells were transiently transfected with the optimized cis-FACTORIALTM library. Twenty-four hours after transfection cells were washed and supplied with fresh low serum (1% FBS, charcoal stripped) culture medium and treated with inducer for 16 h. Profile of the cis-FACTORIALTM activities was determined as fold of induction values versus vehicle-treated (DMSO) control cells. The graph shows fold-induction data plotted on a logarithmic scale. Insert table is a list of transcriptional pathways tested for their activity. (G) Wnt signaling modulation of salpn in zebrafish. (E) Wnt reporter activity was visualized in zebrafish using whole-mount in situ hybridization (WISH) for GFP expression.