Fig. 2

LGP2 promotes IFN response at the early phase of SVCV infection but attenuates at the late phase

(A) mRNA expression comparison of two ifn genes and three ISGs between lgp2^+/+ and lgp2^lof/lof zebrafish larvae following SVCV infection. Left panels: lgp2^+/+ and lgp2^lof/lof zebrafish larvae (6 dpf) were immersed with SVCV (5×10⁵ TCID₅₀/mL) for the indicated time points, followed by RT-PCR detection of the mRNA levels of ifnφ1, ifnφ3, mxb, irf3, and irf7. The expression values were expressed as fold induction relative to that in mock-infected larvae following normalization to β-actin. Right panels: the induction ratio of each gene between lgp2^lof/lof and WT larvae was calculated based on the fold induction at the same time point, followed by normalization to the ratio at 0 h postinfection, which was set to 1, indicating no change.

(B) mRNA expression comparison of two ifn genes and four ISGs in spleens between lgp2^+/+ and lgp2^lof/lof zebrafish adults following SVCV infection. Left panels: lgp2^+/+ and lgp2^lof/lof zebrafish adults (60 dpf) were injected i.p. with SVCV (10⁸ TCID₅₀/mL) for the indicated time points, followed by RT-PCR detection. Right panels: the fold induction ratios of each gene in spleen between lgp2^+/+ and lgp2^lof/lof zebrafish adults. Data were shown as mean ± SD (N = 3). P values were calculated using Student’s t test. ∗p < 0.05, ∗∗p < 0.01; n.s., not significant. See also Figure S2, and Table S1.