E2 rescue the hepatic steatosis and the AKT-mTOR signaling activity in liver. (A, B) Relative expression of gh1 gene in pitutary (A) of igf1-deficient and wild type treated in E2(100μg/L). Triglyceride composition in liver (B) of igf1-deficient and wild type treated in E2(100μg/L). An independent experiment was carried out in which the control group zebrafish was treated with 5% DMSO in fish water. (C, D) Western blot analysis of hepatic AKT-mTOR signal activity in male zebrafish. Four animals were sampled for each experiment and three biological repeats were carried out and statistical analysis was performed using a t test (p<0.001, n=3). *p < 0.05, ***P < 0.001.
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