FIGURE

Fig. 3

ID

ZDB-FIG-220623-65

Publication

Silvennoinen et al., 2022 - SCN1A overexpression, associated with a genomic region marked by a risk variant for a common epilepsy, raises seizure susceptibility

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Fig. 3

Overexpression of scn1a in zebrafish increases seizure susceptibility and temperature sensitivity. a Representative images of control (Ctrl; left panel) and scn1lab-OE larvae (right panel). Red colour depicts mCherry fluorescence. b Number of EEG discharges in control and scn1lab-OE larvae. Horizontal bars represent mean ± SEM. Sample sizes: Ctrl, n = 8; scn1lab-OE + Veh, n = 19; scn1lab-OE + valproic acid (VPA) (100 µM), n = 8; scn1lab-OE + phenytoin (PHT) (100 µM), n = 15; scn1lab-OE + oxcarbazepine (OXC) (170 µM), n = 9. **P < 0.01, *P < 0.05 (One-way ANOVA with Tukey’s post-hoc). Abbreviations: VPA-valproic acid, PHT-phenytoin, OXC- oxcarbazepine. c An example of EEG recordings obtained from the optic tecta of 4 dpf scn1lab-OE larvae. d Number of discharges recorded in scn1lab-OE larvae relative to scn1lab mRNA levels. Vertical bars represent mean ± SEM. e Percentage of larvae developing heat-induced seizures. Sample sizes: Ctrl, n = 18, n = 20 or n = 22 (for 33, 35 or 37 °C, respectively); scn1lab-OE, n = 16, n = 19 or n = 18 (for 33, 35 or 37 °C, respectively). ***P < 0.001, **P < 0.01 (Two-way ANOVA with Sidak’s multiple comparisons test)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf3628Tg (AB)
Condition:	heat shock
Observed In:	involuntary skeletal muscle contraction neuronal action potential propagation regulation of transmission of nerve impulse whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Acta Neuropathol.