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Fig. 3
Overexpression of scn1a in zebrafish increases seizure susceptibility and temperature sensitivity. a Representative images of control (Ctrl; left panel) and scn1lab-OE larvae (right panel). Red colour depicts mCherry fluorescence. b Number of EEG discharges in control and scn1lab-OE larvae. Horizontal bars represent mean?±?SEM. Sample sizes: Ctrl, n?=?8; scn1lab-OE?+?Veh, n?=?19; scn1lab-OE?+?valproic acid (VPA) (100 µM), n?=?8; scn1lab-OE?+?phenytoin (PHT) (100 µM), n?=?15; scn1lab-OE?+?oxcarbazepine (OXC) (170 µM), n?=?9. **P?<?0.01, *P?<?0.05 (One-way ANOVA with Tukey?s post-hoc). Abbreviations: VPA-valproic acid, PHT-phenytoin, OXC- oxcarbazepine. c An example of EEG recordings obtained from the optic tecta of 4 dpf scn1lab-OE larvae. d Number of discharges recorded in scn1lab-OE larvae relative to scn1lab mRNA levels. Vertical bars represent mean?±?SEM. e Percentage of larvae developing heat-induced seizures. Sample sizes: Ctrl, n?=?18, n?=?20 or n?=?22 (for 33, 35 or 37 °C, respectively); scn1lab-OE, n?=?16, n?=?19 or n?=?18 (for 33, 35 or 37 °C, respectively). ***P?<?0.001, **P?<?0.01 (Two-way ANOVA with Sidak?s multiple comparisons test)