Fig. 5

Dnmt1 represses Notch signaling to regulate HSPC generation. (A) qPCR analysis showing expression of Notch-related genes notch1a, notch1b, notch2, notch3, dll4, hey2, her1 and her15.1 in kdrl⁺/runx1⁺ HECs. n=3 replicates. (B) Protein level of Notch1 in control and dnmt1-deficient embryos at 36?hpf. (C) Confocal imaging showing the number of tp1⁺/fli1a⁺ cells in the AGM in control and dnmt1 morphants (white arrowheads, upper panels) with quantification (bottom panel). n?3 replicates. (D) WISH analysis showing the expression of runx1 and cmyb (arrowheads) at 36?hpf in control and dnmt1 morphants treated with DMSO or DBZ. n?3 replicates. (E) Statistical analysis of the WISH data in D. (F) qPCR analysis of runx1 and cmyb expression in control and dnmt1 morphants treated with DMSO or DBZ at 36?hpf. n=3 replicates. Data are mean±s.d. **P<0.01, ***P<0.001, ****P<0.0001 (unpaired two-tailed Student's t-test). NS, no significance. Scale bars: 50 µm (C); 100?µm (D).