Fig. 7

Modulation of PPAR signaling impacts pancreatic gene expression. A) Gene expression of endocrine (gcga, insa, pdx1) and exocrine (try, amy2a) pancreas transcripts was assessed following PPARβ/δ modulation using qPCR. Agonism of PPARβ/δ increases gcga expression by almost 10-fold, and decreases expression of pdx1 and try, indicative of potential endocrine and exocrine effects. B) Putative PPAR transcription factor binding sites (TFBS) were examined in the promoters of pancreatic gene targets. Each gene had several isoform-specific or multiple isoform binding sites in the promoter or gene body, including several directly adjacent to the transcription start site. Asterisks (*) indicate a significant difference between controls and a PPAR modulator, while octothorpes (#) indicate a significant difference between the agonist and antagonist. α = 0.05, n = 6–10 samples per group each containing 8–10 pooled embryos.