IMAGE

Figure 4

ID

ZDB-IMAGE-220416-26

Genes

Publication

Huang et al., 2022 - Negative Elongation Factor (NELF) Inhibits Premature Granulocytic Development in Zebrafish

All Figures

Figures for Huang et al., 2022

Image

Figure Caption

Figure 4

Expression of human NELFB in hematopoietic cells partially rescues granulopoiesis in nelfb^−/− embryos. (A) Alignment of human, mouse, and zebrafish NELFB proteins. Identical and similar amino acids are shaded in gray. (B) Generation of Tg(drl:hNELFB-2A-GFP) fish and GFP expression. (C) WISH for scl or pu.1 in WT and Tg(drl:hNELFB-2A-GFP) embryos at 22 hpf. (D) Quantification of WISH results in (C) (n = 40–60 embryos per group). (E) WISH for mpx in WT, Tg(drl:hNELFB-2A-GFP), nelfb^−/− and nelfb^−/−; Tg(drl:hNELFB-2A-GFP) embryos at 22 hpf. (F) Quantification of WISH results in (E) (n = 20–30 embryos per group). All results are presented as the mean ± SD from three independent experiments (t test, * for p < 0.05). “+++” and “+” in (D,F) respectively represent strong staining and weak staining. hNELFB, human NELFB.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.