FIGURE

Figure 7.

ID

ZDB-FIG-220323-11

Publication

Okada et al., 2022 - Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

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Figure 7.

Predictive power of current CRISPR-Cas9 gRNA design tools for dgRNA/Cas9 RNPs. The heat map shows Spearman rank correlation coefficients (r_s) between prediction scores of major gRNA design tools for 51 crRNAs tested in this study (Supplementary Tables S3 and S7) and ICE indel frequency values for WT (left) and HiFi (right) Cas9 (Supplementary Table S4). Corresponding scatter plots are shown in Supplementary Figure 3. For DeepSpCas9variants, tRNA-N20 sgRNA values were used.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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