FIGURE

Figure 5.

ID

ZDB-FIG-220323-9

Publication

Okada et al., 2022 - Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

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Figure 5.

Position-specific mononucleotide features determined with kpLogo. (A) Nucleotide frequency at each position of input sequences. Position 1 is assigned to the 5′-terminal nucleotide of the 20-nt protospacer target sequence (positions 1–20) and positions 21–23 correspond to PAM. (B) A kpLogo plot of mononucleotide features obtained for WT Cas9. Favored and disfavored nucleotides are shown in the upper and lower sides, respectively, with the height scaled to its P-value (–log₁₀ transformed) derived from Student's t tests of whether crRNAs with a particular nucleotide at a specific position were more or less efficient than other crRNAs. (C) A kpLogo plot of mononucleotide features obtained for HiFi Cas9. Significant nucleotides after Bonferroni correction done at each position are marked in (B) and (C) (**P < 0.01/4 = 0.0025; *P < 0.05/4 = 0.0125).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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