FIGURE

Figure 3.

ID

ZDB-FIG-220323-7

Publication

Okada et al., 2022 - Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

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Figure 3.

Dose-dependent cleavage efficiencies of selected crRNAs from low, medium, and high activity groups. Two crRNAs each from high (A), medium (B) and low (C) activity groups were injected as RNP at four different amounts of 0.75, 1.5, 3 and 4.5 fmol. Indel frequencies (in %) assessed with ICE for RNP complexes assembled with WT Cas9 and HiFi Cas9 are plotted on the left and right sides, respectively. Means of replicates are shown with standard error bars. The experimental dataset is shown in Supplementary Table S5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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