FIGURE

Figure 2.

ID

ZDB-FIG-220323-6

Publication

Okada et al., 2022 - Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

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Figure 2.

Cleavage efficiencies of the 51 crRNAs in the dgRNA RNP complex assembled with WT or HiFi Cas9. (A) A scatter plot of indel frequencies (in %) of the 51 crRNAs assessed with TIDE and ICE with the use of WT Cas9. (B) A scatter plot of indel frequencies of the 51 crRNAs assessed with TIDE and ICE using HiFi Cas9. (C) Comparison of indel frequencies generated with WT and HiFi Cas9 RNP complexes and assessed with ICE. Pearson correlation coefficients (r_p) are shown in each panel (A–C). (D) Indel frequencies (in %) assessed with ICE for the 51 crRNAs in dgRNA RNP complexes assembled with WT Cas9 and HiFi Cas9 are plotted on the left and right sides, respectively. Means of replicates are shown with the standard error bars. The experimental dataset for (A)–(D) is shown in Supplementary Table S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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