ZFIN ID: ZDB-FIG-220319-57
Liu et al., 2022 - Rod genesis driven by mafba in an nrl knockout zebrafish model with altered photoreceptor composition and progressive retinal degeneration. PLoS Genetics   18:e1009841 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Days 7-13 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Adult

Fig 8 Identification of the <italic toggle='yes'>mafba</italic> gene as a novel driving factor for the development of <italic toggle='yes'>nrl</italic>-independent rods.

(A) Clustering analysis of the large MAF genes and the genes involved in photoreceptor development by using the expression data from scRNA-seq. The cell-type-specific expression pattern of mafba was highly similar to those of nrl and nr2e3. (B) The distribution of rods (labeled with EGFP) in the nrl-KO zebrafish carrying the WT, heterozygous, and homozygous mafba alleles at 9 dpf (left panel) and 15 dpf (right panel). Knocking out mafba further reduced the genesis of rods in the nrl-KO zebrafish. The dotted circles indicate the boundaries of the retinas. Scale bars: 100 μm. (C) The expression levels of nrl, nr2e3, and mafba in the nrl-KO retinas at 2 mpf and 5 mpf were measured via RNA-seq and qPCR. The data are shown as mean with SD (n = 3). *, p < 0.05; **, p < 0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP mafbahzu17/+; nrlhzu16/hzu16; kj2Tg standard conditions Days 7-13 retinal rod cell IFL
Days 14-20 retinal rod cell IFL
mafbahzu17/hzu17; nrlhzu16/hzu16; kj2Tg standard conditions Days 14-20 Not Detected retinal rod cell IFL
nrlhzu16/hzu16; kj2Tg standard conditions Days 7-13 retinal rod cell IFL
Days 14-20 retinal rod cell IFL
mafba WT standard conditions Days 45-89 eye RTPCR
Days 45-89 eye RNA-Seq
Adult eye RTPCR
nrlhzu16/hzu16 standard conditions Days 45-89 eye RTPCR
Days 45-89 eye RNA-Seq
Adult eye RTPCR
nr2e3 WT standard conditions Days 45-89 eye RTPCR
Days 45-89 eye RNA-Seq
Adult eye RTPCR
nrlhzu16/hzu16 standard conditions Days 45-89 eye RNA-Seq
Days 45-89 eye RTPCR
Adult eye RTPCR
nrl WT standard conditions Days 45-89 eye RNA-Seq
Days 45-89 eye RTPCR
Adult eye RTPCR
nrlhzu16/hzu16 standard conditions Days 45-89 eye RTPCR
Days 45-89 eye RNA-Seq
Adult eye RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mafbahzu17/+; nrlhzu16/hzu16; kj2Tg standard conditions Days 7-13 retinal rod cell decreased amount, abnormal
Days 7-13 retinal rod cell EGFP expression decreased distribution, abnormal
Days 14-20 retinal rod cell decreased amount, abnormal
Days 14-20 retinal rod cell EGFP expression decreased distribution, abnormal
mafbahzu17/hzu17; nrlhzu16/hzu16; kj2Tg standard conditions Days 7-13 retinal rod cell absence of anatomical entity, abnormal
Days 14-20 retinal rod cell EGFP expression absent, abnormal
nrlhzu16/hzu16 standard conditions Days 45-89 eye mafba expression decreased amount, abnormal
Days 45-89 eye mafba expression decreased amount, abnormal
Days 45-89 eye nrl expression increased amount, abnormal
Adult eye mafba expression decreased amount, abnormal
Adult eye nr2e3 expression decreased amount, abnormal
Adult eye nrl expression increased amount, abnormal
nrlhzu16/hzu16; kj2Tg standard conditions Days 7-13 retinal rod cell decreased amount, abnormal
Days 7-13 retinal rod cell EGFP expression decreased distribution, abnormal
Days 14-20 retinal rod cell decreased amount, abnormal
Days 14-20 retinal rod cell EGFP expression decreased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.