ZFIN ID: ZDB-FIG-220319-59
Liu et al., 2022 - Rod genesis driven by mafba in an nrl knockout zebrafish model with altered photoreceptor composition and progressive retinal degeneration. PLoS Genetics   18:e1009841 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Adult

Fig 10 Progressive degeneration and regeneration in the <italic toggle='yes'>nrl</italic>-KO retinas.

(A) The TUNEL assay results revealed that multiple types of retinal cells, including rods, cones, RPE, and inner retinal cells, undergo apoptosis in the nrl-KO retinas. Representative images are shown. Scale bar: 20 μm. (B) Quantitation of the apoptotic cells per section from 5 mpf to 13 mpf. The results are shown as mean with SD (n = 3). *, p < 0.05; **, p < 0.01. (C) RPE morphology in the WT and nrl-KO zebrafish at 20 mpf are shown, as assessed by immunostaining the retinal whole-mounts for ZO-1 Scale bar: 20 μm. (D) The up-regulation of GFAP in nrl-KO retinas as detected by immunostaining. CN, cone nuclear layer; RN, rod nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 25 μm. (E) Regeneration of photoreceptors was more active in the nrl-KO retinas at 9 mpf and 18 mpf, as reflected by the increase in the number of Pcna-positive cells (proliferating cells) in the ONL, compared with the WT levels. Scale bars: 50 μm. (F) Quantitation of the Pcna+ cells located in the ONL per section. The results are shown as mean with SD (n = 4) from 7 dpf to 29 mpf. *, p < 0.05; **, p < 0.01.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab7-tjp1 IHC WT standard conditions Adult retinal pigmented epithelium
IHC nrlhzu16/hzu16 standard conditions Adult retinal pigmented epithelium
Ab17-pcna IHC WT standard conditions Adult retinal photoreceptor layer
IHC nrlhzu16/hzu16 standard conditions Adult retinal photoreceptor layer
Ab22-gfap IHC WT standard conditions Adult retinal ganglion cell layer
IHC nrlhzu16/hzu16 standard conditions Adult retinal ganglion cell layer
Phenotype Details
Fish Conditions Stage Phenotype
nrlhzu16/hzu16 standard conditions Days 7-13 retinal photoreceptor layer cell population proliferation normal process quality, normal
Days 30-44 retinal photoreceptor layer cell population proliferation normal process quality, normal
Days 45-89 retinal photoreceptor layer cell population proliferation normal process quality, normal
Adult retinal cone cell apoptotic process increased process quality, abnormal
Adult retinal ganglion cell layer Ab22-gfap labeling increased distribution, abnormal
Adult retinal inner nuclear layer apoptotic process increased process quality, abnormal
Adult retinal photoreceptor layer Ab17-pcna labeling increased distribution, abnormal
Adult retinal photoreceptor layer cell population proliferation increased process quality, abnormal
Adult retinal pigmented epithelium apoptotic process increased process quality, abnormal
Adult retinal rod cell apoptotic process increased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.