Figure 2

Measurements of cartilaginous structures of the zebrafish jaw as assessed by Alcian blue staining. (A) Sagittal view of the head: P—palatoquadrate cartilage, M—Meckels cartilage, d—distance (mm) from P to M, e—extension of M past P. (B) Ventral view of the head: L1—length of Meckels cartilage (mm), L2—Length of ceratohyal cartilage (mm), length of the cartilages was estimated using the Pythagorean formula L = √ (s2 − b2/4). (C) Ventral view of the head: ch—angle of the ceratohyal cartilage. Data for angle of ceratohyal (degrees) (D), ceratohyal length (mm) (E), Meckel’s length (mm) (F) and distance from palatoquadrate to Meckel’s cartilage (mm) (G). Treatment groups; untreated controls, 5 µM AFN and 5 µM AFN with concurrent RBC exposure (6 hpf–5 dpf). Error bars show the standard error of the mean. Mean is the average of six larvae for each treatment group. Asterisk indicates values that are statistically significantly different (p < 0.01) as assessed by one-way ANOVA, Tukey post-hoc analysis. All linear measurements are in mm. Auranofin, AFN; Riboceine, RBC; hours post fertilization, hpf; days post fertilization, dpf.