PUBLICATION
Riboceine Rescues Auranofin-Induced Craniofacial Defects in Zebrafish
- Authors
- Leask, M., Carleton, C., Leeke, B., Newman, T., Antoun, J., Farella, M., Horsfield, J.
- ID
- ZDB-PUB-211230-4
- Date
- 2021
- Source
- Antioxidants (Basel, Switzerland) 10(12): (Journal)
- Registered Authors
- Horsfield, Jules
- Keywords
- Riboceine, antioxidant, auranofin, craniofacial anomalies, neural crest, oxidative stress, zebrafish
- MeSH Terms
- none
- PubMed
- 34943067 Full text @ Antioxidants (Basel)
Citation
Leask, M., Carleton, C., Leeke, B., Newman, T., Antoun, J., Farella, M., Horsfield, J. (2021) Riboceine Rescues Auranofin-Induced Craniofacial Defects in Zebrafish. Antioxidants (Basel, Switzerland). 10(12):.
Abstract
Craniofacial abnormalities are a common group of congenital developmental disorders that can require intensive oral surgery as part of their treatment. Neural crest cells (NCCs) contribute to the facial structures; however, they are extremely sensitive to high levels of oxidative stress, which result in craniofacial abnormalities under perturbed developmental environments. The oxidative stress-inducing compound auranofin (AFN) disrupts craniofacial development in wildtype zebrafish embryos. Here, we tested whether the antioxidant Riboceine (RBC) rescues craniofacial defects arising from exposure to AFN. RBC rescued AFN-induced cellular apoptosis and distinct defects of the cranial cartilage in zebrafish larvae. Zebrafish embryos exposed to AFN have higher expression of antioxidant genes gstp1 and prxd1, with RBC treatment partially rescuing these gene expression profiles. Our data suggest that antioxidants may have utility in preventing defects in the craniofacial cartilage owing to environmental or genetic risk, perhaps by enhancing cell survival.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping