FIGURE

FIGURE 3

ID

ZDB-FIG-220131-452

Publication

Khan et al., 2021 - Carbofuran affects cellular autophagy and developmental senescence through the impairment of Nrf2 signalling

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FIGURE 3

Carbofuran affects Nrf2 signalling. (A) Schematic illustration of the Nrf2-ARE signalling pathway as well as monitoring the pathway using a transgenic model. Nrf2 translocates into the nucleus by releasing Keap1. In the nucleus, Nrf2 transactivates target genes including gstp1 through binding to ARE regulatory element. The Nrf2-ARE signalling pathway was monitored using a double-transgenic zebrafish line expressing Neh2 domain (of Nrf2)-mCherry and gstp1-EGFP. (B) Effect of carbofuran on the Nrf2-ARE signalling pathway. Embryos were treated with carbofuran for 24 hours, started from 56 hpf and observed under both bright field (BF) (top row) and fluorescence conditions (bottom two rows) of the microscope. Both Neh2-mCherry and gstp1-EGFP expressions were down-regulated by carbofuran exposure. Scale bar: 250 μm. The number of animals was 10 (n = 10). Data are presented as mean ± SE. **P ≤ .01; ***P ≤ .005

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell. Mol. Med.