FIGURE

Fig. 3

ID

ZDB-FIG-220131-144

Publication

Fabian et al., 2022 - Lifelong single-cell profiling of cranial neural crest diversification in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

a, b, UMAPs at 1.5 dpf generated by scRNAseq versus integration of scRNAseq and snATACseq datasets using SnapATAC. SnapATAC outperforms scRNAseq in resolving dorsoventral (vertical), anterior-posterior (horizontal), and major arch landmarks including a previously unappreciated oral-aboral axis in zebrafish. c Select enriched transcription factor binding motifs for each cluster. d Gene body activities for transcription factors and their corresponding DNA-binding motifs reveal tight correspondence with published expression patterns, including zebrafish-specific overlap of dlx5a and hand2 in the mandibular arch (arrow). e Fluorescent in situ hybridization shows restricted expression of nr5a2 in the aboral domain of the mandibular arch as predicted by SnapATAC. sox10:dsRed labels CNCCs of the arches in blue (numbered, anti-dsRed antibody stain). Scale bar, 20 μm.

Expression Data

Genes:	dlx5a hand2 meis2b nr5a2 pitx1 pou3f3b
Fish:	el10Tg/el10Tg; sd27Tg/sd27Tg
Anatomical Terms:	dental organ mandibular arch skeleton neural crest cell opercle quadrate-anguloarticular joint
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	el10Tg/el10Tg; sd27Tg/sd27Tg
Observed In:	neural crest cell differentiation
Stage:	Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.