FIGURE

Figure 3.

ID

ZDB-FIG-220131-137

Publication

Tatarakis et al., 2021 - Single-cell transcriptomic analysis of zebrafish cranial neural crest reveals spatiotemporal regulation of lineage decisions during development

Other Figures

All Figure Page

Back to All Figure Page

Figure 3.

Pseudotemporal analysis reveals emergence of NC lineages during migration

(A) ForceAtlas (FA) embedding of single-cell timeline initialized using PAGA graph with time point identities of individual cells labeled and color coded.

(B) Module scores, color code distinct from (A), for early NC, pigment, skeletal, and neural and glial lineages overlaid on FA embedding.

(C) CellAssign probabilistic cell type labels computed using module score genes overlaid on FA embedding. Color code is distinct from (A) or (B).

(D) Pseudotime calculated using PAGA graph. Cells are arranged according to pseudotime values along the x axis, with pseudotime scaled within each branch. Branch points are split on the y axis and connected by dashed lines. Color code corresponds to (C).

(E) Scatterplots showing the increased specificity of marker genes to lineages across developmental time. Each point represents a gene contributing to a lineage score (color code from C) with the axes showing its Pearson correlation to the total score for pigment, skeletal, or neural and glial across all cells in each time point.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.