FIGURE

Fig. 8

ID

ZDB-FIG-220124-29

Publication

Taler et al., 2019 - Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate

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Fig. 8

Inhibition of TGFβ signaling restores normal expression of pSmad3, Prox1 and Aquaporin 0. (A–F) Confocal images of sections from eyes of 4 dpf plod3^vu222/vu222 embryos treated with vehicle (DMSO) (A,C,E) or with SB-431542 (B,D,F) to inhibit TGFβ signaling via TGF type I receptor. Sections were labeled for pSmad3 (red, A,B), Prox1 (red, C,D) and Aq0 (red, E,F). In all panels, nuclei are labeled with DAPI (blue). Arrows in A,C,E point at abnormal expression of pSmad3, Prox1 and Aq0, respectively, in the cell masses. Data is representative of the following number of eyes: A = 9, B = 11, C = 6, D = 12, E = 7, F = 10. L, lens. Scale bars are 20 μm.

Expression Data

Antibodies:	Ab1-mip Ab2-smad3 Ab13-prox1
Fish:	plod3^vu222/vu222
Condition:	chemical treatment by environment: SB 431542
Anatomical Term:	lens epithelium
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plod3^vu222/vu222
Condition:	chemical treatment by environment: SB 431542
Observed In:	lens epithelium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 458(2), Taler, K., Weiss, O., Rotem, S., Rubinstein, A.M., Seritrakul, P., Gross, J.M., Inbal, A., Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate, 177-188, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.