FIGURE

Fig. 7

ID

ZDB-FIG-200413-41

Publication

Taler et al., 2019 - Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate

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Fig. 7

TGFβ signaling drives formation of cellular masses in plod3-mutant lenses. (A,B) Single confocal planes of eyes from 75 hpf normal (A) and plod3^vu222/vu222(B) embryos labeled for pSmad3 (red) and nuclei (DAPI, blue). Numbers of eyes analyzed: A = 8, B = 10. Arrowheads in B point at the abnormal ALE that shows expression of pSmad3, and insets in A and B show pSmad3 channel alone in the ALE region. (C–F) Live 4 dpf plod3^vu222/vu222 embryos (C,D,F) or normal sibling (E), dorsal views, anterior to the top. Embryos in C,D,E were treated from 30 hpf with vehicle (DMSO) and in F with SB-431542. Arrows point at lenses. (G) Quantification and statistical analysis of treatment of plod3^vu222/vu222 with SB-431542 (χ2 test). Location of lenses relative to retina is represented by different colors. (H,I) Transverse histological sections from eyes of plod3^vu222/vu222 embryos at 4 dpf. Embryo in H was treated with vehicle and in I with SB-431542. Ten eyes were analyzed in two separate experiments. Arrows in H point at cellular mass and in I at rescued monolayer of ALE with abnormal cells. Scale bars are 20 μm (A,B,H,I) or 100 μm (C–F).

Expression Data

Antibody:	Ab2-smad3
Fish:	WT plod3^vu222/vu222
Anatomical Term:	lens epithelium
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plod3^vu222/vu222
Condition:	chemical treatment by environment: SB 431542
Observed In:	lens lens epithelium lens epithelium anterior region lens epithelium transforming growth factor beta receptor signaling pathway
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 458(2), Taler, K., Weiss, O., Rotem, S., Rubinstein, A.M., Seritrakul, P., Gross, J.M., Inbal, A., Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate, 177-188, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.