Figure 5

Reduced blastoderm cell cohesion in MZlurap1 mutant embryos. (a?d) Representative images from hang drop assay show differential affinity between wild-type and MZlurap1 mutant cells, as reflected by the preferential aggregation of cells with the same genotypes. Scale bar: 200 µm. (e?h) Representative images show cluster formation in wild-type and MZlurap1 mutant cells on fibronectin-coated substrates. At 3 h and 6 h, clusters consisted of at least 4 cells in close contact are encircled by dashed lines. Scale bar: 200 µm. (i,j) Statistical analyses compare the number and the size of cell clusters formed by wild-type and MZlurap1 mutant cells at 3 h and 6 h. Data were collected from 6 randomly selected view fields for each condition. Bars represent the mean value ± s.d. (*, i>p < 0.05; ***, p < 0.001; ****, p < 0.0001).