Figure 3

Delayed epibloy progression and reduced spreading capacity of DEL cells in MZlurap1 mutant embryos. (a) Phalloidin and DAPI staining of wild-type and MZlurap1 mutant embryos at 80% epiboly. Scale bar: 100 ?m. (b) Statistical analysis of the extent of vegetal movements of the EVL and the DEL in wild-type and MZlurap1 mutant embryos. The percentage of epiboly was determined as the distance from the animal pole to the front of the EVL or the DEL margin with respect to the distance between the animal pole and the vegetal pole. Data were collected from 8 to 9 wild-type or MZlurap1 mutant embryos. Bar represents the mean value ± s.d. (NS, not significant; *, p < 0.05). (c) UV-induced green to red fluorescence conversion of Kaede GFP in the animal pole region at 4 hpf. Areas within dashed circles reflect the extent of DEL cell spreading at indicated times. Scale bar: 100 ?m. (d) Statistical analysis of the extent of cell spreading. Data were collected from 6 embryos for each condition in three independent experiments, and the area initially occupied by labeled cells in wild-type embryos is normalized as 1. Bars represent the mean value ± s.d. (NS, not significant; *, p < 0.05; ***, p < 0.001). (e) Representative images of mGFP-labeled wild-type cells or MZlurap1 mutant cells transplanted to unlabeled wild-type recipients. Scale bar: 10 µm.