Fig. 5

a Oogenesis of D. melanogaster. Upon protein starvation, either germ cells or stage 8/9 egg chambers are degenerated. The previtellogenic egg chambers slow down the growth, then resume the growth within 2 h once protein is available. During egg chamber growth, meiotic chromosomes arrest at the prophase I until stage 13 (blue lines). b The number of previtellogenic egg chambers (stages 2?7) per fly are similar between wild-type (w¹¹¹⁸) female virgin controls (n = 13 animals) and the octopamine mutant female virgins (tbh^?/? (n = 11 animals) and oct?2R^?/? (n = 14 animals)). Two-sided t-test: p = 0.63 (w¹¹¹⁸ vs. tbh^?/?), 0.22 (w¹¹¹⁸ vs. oct?2R^?/?), 0.12 (tbh^?/? vs. oct?2R^?/?). Canton-S (n = 35) has fewer number of egg chambers than w¹¹¹⁸. p = 5.5 × 10^?11 (Canton-S vs. w¹¹¹⁸), 1.7 × 10^?10 (Canton-S vs. tbh^?/?), 1.8 × 10^?8 (Canton-S vs. oct?2R^?/?). n.s.: not significant. c The percent of each stage of egg chambers in the ovaries of tbh^+/? and tbh^?/? virgins during the protein starvation period. The percent of stage 14 egg chambers increases in tbh^?/? mutants compared to that of the heterozygote control, tbh^+/?. These data are extracted from the raw data shown in d?f. d?f The distribution of each stage egg chambers in the ovaries of each tbh^+/? (n = 29 animals for 1 dpe, 14 animals for 1.5 dpe, 59 animals for 2 dpe) and tbh^?/? (n = 32 animals for 1 dpe; 17 animals for 1.5 dpe, 59 animals for 2 dpe) female virgin during the protein starvation period. At each time point, tbh^?/? mutants contain more progressed egg chambers compared to tbh^+/?. b, d?f Boxplots show the median, mean (X), interquartile range (IQR). The upper whisker: the maxima smaller than 1.5 times IQR plus the third quartile, the lower whisker: the minima larger than 1.5 times IQR minus the first quartile. Source data are provided as a Source Data file.