ZFIN Figure: Kim et al., 2021, Fig. 6

Fig. 6

Octopamine regulates quiescence via Octβ2R, competing with nutrients.

a, b Upon protein starvation, tbh^−/− or octβ2R^−/− virgins contain more stage 14 egg chambers (S14, white dotted ellipses) than controls. Representative images (Scale bar = 100 µm) (a) and the quantification (b). Arrows indicate stage 9. n = 85 tbh^−/−; 35 octβ2R^−/−; 137 Canton-S; 52 w¹¹¹⁸; 47 tbh^+/−. Two-sided t-test: p = CS vs. w¹¹¹⁸ (0.0001); vs. tbh^+/− (1.2 × 10⁻⁶); vs. tbh^−/− (8.4 × 10⁻³⁶); vs. octβ2R^−/− (1.4 × 10⁻¹⁰), w¹¹¹⁸ vs. tbh^+/− (0.21); vs. tbh^−/− (2.6 × 10⁻²⁵); vs. octβ2R^−/− (2.3 × 10⁻¹⁰); 7.2 × 10⁻²¹ (tbh^−/− vs. tbh^+/−); 8.8 × 10⁻⁷ (tbh^+/− vs. octβ2R^−/−); 0.070 (tbh^−/− vs. octβ2R^−/−). c Exogenous OA (5 mg/ml) rescues tbh^−/− mutants, but not octβ2R^−/−. n = 85 animals (−OA), 11 animals (+OA) for tbh^−/−; 35 animals (−OA), 9 animals (+OA) for octβ2R^−/−; 7 animals (+OA) for tbh^+/−. Two-sided t-test: p = 7.5 × 10⁻⁹ for tbh^−/−, 0.77 for octβ2R^−/− between untreated and treated with OA. The same data for without OA in Fig. 6b was replotted. d Without nutrients, controls produce few S14 (n = 25 Canton-S, 11 w¹¹¹⁸), whereas tbh^−/− (n = 99 animals) produces at a similar rate to that of Canton-S fed on CSY. tbh^+/− (n = 116 animals) produces S14 ranging between wild-type and tbh^−/−. Two-sided t-test: p = CS vs. w¹¹¹⁸ (0.05), vs. tbh^+/− (3.1 × 10⁻⁶), vs. tbh^−/− (1.7 × 10⁻¹⁰), w¹¹¹⁸ vs. tbh^+/− (0.008), vs. tbh^−/− (4.4 × 10⁻⁵), 9.4 × 10⁻¹⁰ (tbh^+/− vs. tbh^−/−). e Exogenous OA restores oocyte quiescence to tbh^+/− and tbh^−/− in a concentration dependent manner. n = 33 tbh^+/−, 28 tbh^−/− for 5 mg/ml OA, 43 tbh^+/−, 26 tbh^−/− for 10 mg/ml OA. Two-sided t-test: p = 9.4 × 10⁻¹⁰ (tbh^+/− vs. tbh^−/−), 0.11 (tbh^+/−: OA0 vs. OA5), 0.0005 (tbh^+/−: OA0 vs. OA10), 0.0013 (tbh^−/−: OA0 vs. OA5), 0.0001 (tbh^−/−: OA0 vs. OA10). f OA and nutrients compete in regulation of egg chamber growth. n = 55 Canton-S (CSY1%), 73 Canton-S (1% + OA5), 107 Canton-S (CSY2.5%), 79 Canton-S (CSY2.5% + OA5), 53 Canton-S (CSY2.5% + OA10). Two-sided t-test: p = 0.0006 (CSY1% vs. CSY1% + OA5), 0.21 (CSY2.5% vs. CSY2.5% + OA5), 1.4 × 10⁻⁷ (CSY2.5% vs. CSY2.5% + OA10). b–f Numbers of egg chambers per fly were shown. Boxplots show the median, mean (X), interquartile range (IQR). The upper whisker: the maxima smaller than 1.5 times IQR plus the third quartile, the lower whisker: the minima larger than 1.5 times IQR minus the first quartile. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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