ZFIN Figure: Kim et al., 2021, Fig. 6

Fig. 6

Octopamine regulates quiescence via Oct?2R, competing with nutrients.

a, b Upon protein starvation, tbh^?/? or oct?2R^?/? virgins contain more stage 14 egg chambers (S14, white dotted ellipses) than controls. Representative images (Scale bar = 100 �m) (a) and the quantification (b). Arrows indicate stage 9. n = 85 tbh^?/?; 35 oct?2R^?/?; 137 Canton-S; 52 w¹¹¹⁸; 47 tbh^+/?. Two-sided t-test: p = CS vs. w¹¹¹⁸ (0.0001); vs. tbh^+/? (1.2 � 10^?6); vs. tbh^?/? (8.4 � 10^?36); vs. oct?2R^?/? (1.4 � 10^?10), w¹¹¹⁸ vs. tbh^+/? (0.21); vs. tbh^?/? (2.6 � 10^?25); vs. oct?2R^?/? (2.3 � 10^?10); 7.2 � 10^?21 (tbh^?/? vs. tbh^+/?); 8.8 � 10^?7 (tbh^+/? vs. oct?2R^?/?); 0.070 (tbh^?/? vs. oct?2R^?/?). c Exogenous OA (5 mg/ml) rescues tbh^?/? mutants, but not oct?2R^?/?. n = 85 animals (?OA), 11 animals (+OA) for tbh^?/?; 35 animals (?OA), 9 animals (+OA) for oct?2R^?/?; 7 animals (+OA) for tbh^+/?. Two-sided t-test: p = 7.5 � 10^?9 for tbh^?/?, 0.77 for oct?2R^?/? between untreated and treated with OA. The same data for without OA in Fig. 6b was replotted. d Without nutrients, controls produce few S14 (n = 25 Canton-S, 11 w¹¹¹⁸), whereas tbh^?/? (n = 99 animals) produces at a similar rate to that of Canton-S fed on CSY. tbh^+/? (n = 116 animals) produces S14 ranging between wild-type and tbh^?/?. Two-sided t-test: p = CS vs. w¹¹¹⁸ (0.05), vs. tbh^+/? (3.1 � 10^?6), vs. tbh^?/? (1.7 � 10^?10), w¹¹¹⁸ vs. tbh^+/? (0.008), vs. tbh^?/? (4.4 � 10^?5), 9.4 � 10^?10 (tbh^+/? vs. tbh^?/?). e Exogenous OA restores oocyte quiescence to tbh^+/? and tbh^?/? in a concentration dependent manner. n = 33 tbh^+/?, 28 tbh^?/? for 5 mg/ml OA, 43 tbh^+/?, 26 tbh^?/? for 10 mg/ml OA. Two-sided t-test: p = 9.4 � 10^?10 (tbh^+/? vs. tbh^?/?), 0.11 (tbh^+/?: OA0 vs. OA5), 0.0005 (tbh^+/?: OA0 vs. OA10), 0.0013 (tbh^?/?: OA0 vs. OA5), 0.0001 (tbh^?/?: OA0 vs. OA10). f OA and nutrients compete in regulation of egg chamber growth. n = 55 Canton-S (CSY1%), 73 Canton-S (1% + OA5), 107 Canton-S (CSY2.5%), 79 Canton-S (CSY2.5% + OA5), 53 Canton-S (CSY2.5% + OA10). Two-sided t-test: p = 0.0006 (CSY1% vs. CSY1% + OA5), 0.21 (CSY2.5% vs. CSY2.5% + OA5), 1.4 � 10^?7 (CSY2.5% vs. CSY2.5% + OA10). b?f Numbers of egg chambers per fly were shown. Boxplots show the median, mean (X), interquartile range (IQR). The upper whisker: the maxima smaller than 1.5 times IQR plus the third quartile, the lower whisker: the minima larger than 1.5 times IQR minus the first quartile. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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