Fig. 2

Cu2+-stressed zebrafish larvae exhibited significantly downregulated expression of gene smyd1b and downregulated level of H3K4me3 protein. A, Expression of methyltransferase complex and histone methyltransferase complex genes in Cu2+-stressed embryos and larvae at 24 hpf. (A1) and 96 hpf (A2), respectively. Expression levels of control were set at 1. B, Trunk muscle cell expression of smyd1b in the control and the Cu2+-stressed embryos at 24 hpf (B1-B4). B5, smyd1b expression level calculated based on WISH data. Each dot represents the signal level in a representative individual embryo in each group. C, Expression of chd8 and ezh2 in the control and the Cu2+-stressed embryos at 24 hpf (C1-C4). C5, chd8 or ezh2 expression level calculated based on WISH data. Each dot represents the signal level in a representative individual embryo in each group. D, Protein levels of H3K27ac, H3K27me3, H3K4me1, and H3K4me3 in the control and the Cu2+-stressed embryos at 24 hpf (D1-D4) and the quantitative analysis of protein level in each sample (D5-D8). Each experiment was repeated two or three times with similar results, and a representative result is shown. B1, B2, C1, C2, lateral view, anterior to the left. C3, C4, dorsal view, anterior to the left. B3, B4, transverse sections for B1, B2, respectively. B1, B2, C1-C4, scale bar, 250 μm. *P < .05, **P < .01, ***P < .001. NS, not significant