FIGURE

Figure 1

ID

ZDB-FIG-211120-109

Publication

Jeong et al., 2021 - Differential Clearance of Aβ Species from the Brain by Brain Lymphatic Endothelial Cells in Zebrafish

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Figure 1

Monomeric A?42 peptides are more efficiently cleared from the brain than oligomeric A?42. (A) A schematic diagram of experimental setup. Fluorescently labeled A?42 prepared at different time points were injected into the brain followed by in vivo imaging at 5 hpi and 24 hpi. (B,C?) Distribution of injected, fluorescently labeled A?42 (mA?42 or oA?42) in the brains of 3 dpf larvae at 5 hpi (B,C) and 24 hpi (B?,C?) (left, overlays with brightfield; middle, HiLyte Fluor 555; right, thresholded images of A?42 fluorescence). (D,E) Quantification of the area fraction (%) occupied by A?42 fluorescence within square unit (512 � 512 pixels) at different time points. (F) Clearance rate of mA?42 and oA?42 between 5 hpi and 24 hpi by fluorescence quantification. Two-tailed unpaired t-test, p = 0.023. Data are presented as mean � SEM. N = 7 per group. Data are representative of at least three independent experiments. dpf, days post fertilization; hpi, hours post injection; HB, hindbrain; OT, optic tectum, Scale bars = 100 ?m. * p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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