FIGURE

Fig. 3

ID

ZDB-FIG-211010-3

Publication

Zhao et al., 2021 - The spliceosome factor sart3 regulates hematopoietic stem/progenitor cell development in zebrafish through the p53 pathway

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Fig. 3

Decreased HSPC proliferation and increased apoptosis in <italic>sart3</italic><sup><italic>smu471</italic></sup> mutants.

A, B Decreased HSPC proliferation in sart3^smu471 mutants. A The BrdU incorporation assay. The white arrowheads indicate proliferating HSPCs (cd41:eGFP^low; BrdU double positive cells). Green indicates eGFP, red indicates BrdU. B Statistical analysis of the percentage of CHT localized cd41^low HSPCs that incorporate BrdU (Student’s t-test, means ± SD; siblings, n = 28; sart3^smu471 mutants, n = 16; ****P < 0.0001). C, D Increased HSPC death in sart3^smu471 mutants. C The TUNEL assay. The white arrowheads indicate HSPCs undergoing cell death (cd41:eGFP^low; TUNEL double positive cells). Green indicates eGFP, red indicates TUNEL. D Statistical analysis of the percentage of CHT localized cd41^low HSPCs labeled by TUNEL (Mann–Whitney U test, means ± SD; siblings, n = 24; sart3^smu471 mutants, n = 27; *P < 0.05). Scale bars: 50 µm.

Expression Data

Antibodies:	Ab8-brdu Ab18-gfp
Fish:	la2Tg sart3^sm471/sm471; la2Tg
Anatomical Terms:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell caudal hematopoietic tissue hematopoietic stem cell
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sart3^sm471/sm471; la2Tg
Observed In:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell caudal hematopoietic tissue hematopoietic stem cell hematopoietic multipotent progenitor cell apoptotic process hematopoietic multipotent progenitor cell cell population proliferation hematopoietic stem cell apoptotic process hematopoietic stem cell cell population proliferation
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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