Fig. 2

atoh8, myoD and crestin expression in early segmentation stages of development (13–14 hpf). a–eatoh8 ISH; f–hatoh8/myoD double ISH. i–katoh8/crestin double ISH. a Flat mount of 13.5-hpf embryo. b 13-hpf embryo showing first appearance of atoh8 in the developing hindbrain (dark arrowhead). c 13.5-hpf embryo. d Embryo at 14 hpf; pink arrowhead indicates the appearance of the second domain at the midbrain/hindbrain boundary. e1–e3 Sections through the 13-hpf embryo at the levels indicated in a. f, g Embryos at 13 hpf and 14 hpf, respectively, double-stained for atoh8 and myoD. h Flat mount of embryo at 14 hpf double-stained for atoh8 and myoD. i, j Flat mount and embryo showing double labelling for crestin and atoh8 at 13 hpf. k Embryo at 14 hpf double-stained for crestin and atoh8. a, h’ Dorsal view, anterior to the top. b–d, f, g, j, k lateral view, anterior to the top, dorsal to the right. h, i dorsal view, anterior to the left. Scale bars: whole embryos 200 µm, flat mounts a, h, i 200 µm; h’ 50 µm; transverse sections: 100 µm