atoh8, atoh8/myoD and atoh8/crestin expression profile at 22 hpf. a–c Whole mounts (lateral view, dorsal to the right) and associated serial transverse sections. d, d’ Flat mounts of zebrafish embryo showing expression in hindbrain, cerebellum and eye (dorsal view, anterior to the left). e Longitudinal sections of the hindbrain and cerebellum reveal the position of atoh8 signal in the superficial layer of the developing neural tissue; atoh8-positive streams of cells can be detected adjacent to the otic vesicle (black arrowhead); there is no clarity about the tissue type of these cells, since they seem to reside in areas apparently marked by crestin transcription (green arrowheads). f Transverse section through the diencephalon demonstrating atoh8 in the lens primordium and part of the retina (upper panel), and crestin at the temporal margin of the developing eye (lower panel). Scale bars: whole embryos 200 µm, flat mounts 100 µm, transverse sections 100 µm
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