atoh8, myoD and crestin expression profile in the 16–16.5-hpf embryo. a, b, f Whole embryos at 16.5 hpf, showing atoh8 and atoh8/myoD and atoh8/crestin signal distribution, respectively; lateral view, dorsal to the right. Arrowheads point at expression in areas of the developing eye, hindbrain and neural crest; anterior to the top. a1–a6 Transverse sections of embryo in (a) at the respective levels; dorsal to the top. c Schematic diagram of atoh8 distribution in brain tissue. d Flat mount of a 16-hpf embryo showing atoh8 expression; dorsal view, anterior to the left. Flat mount (anterior to the left) of 16-hpf embryo double-stained for atoh8 and myoD. e, e1–e3 transverse sections (dorsal to the top) through levels indicated in e. f, gatoh8/crestin signal distribution; arrowheads indicating presence/absence of atoh8 transcripts (violet) and crestin transcripts (red). h Whole embryo showing crestin expression in the head region; dorsal view, anterior to the left. Scale bars: whole embryos, flat mounts 200 µm; transverse sections 100 µm
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