FIGURE

Figure 4

ID
ZDB-FIG-210915-91
Publication
Schellens et al., 2021 - Zebrafish as a Model to Evaluate a CRISPR/Cas9-Based Exon Excision Approach as a Future Treatment Option for EYS-Associated Retinitis Pigmentosa
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Figure 4

Transcript analysis of eysΔexon40-44 mRNA. (A) RT-PCR analysis revealed the absence of eys exons 40-44 in eysΔexon40-44 larvae (5 days post fertilization (dpf)), in the absence of any clear alternatively spliced eys transcripts. (B) Sanger sequencing of the eysΔexon40-44 amplicon confirmed the absence of exons 40-44 from the transcript. (C) Long-range PCR analysis did not reveal any additional and unwanted amplicons derived from CRISPR/Cas9-induced alternative splicing of eys transcripts. PCR (-): negative PCR control.
Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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