Figure 3
- ID
- ZDB-FIG-210915-90
- Publication
- Schellens et al., 2021 - Zebrafish as a Model to Evaluate a CRISPR/Cas9-Based Exon Excision Approach as a Future Treatment Option for EYS-Associated Retinitis Pigmentosa
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Characterization of the stable eys?exon40-44 zebrafish line. (A) Schematic representation of exon excision approach (upper panel). Sanger sequencing confirmed the presence of the anticipated exons 40-44 excision in injected embryos (1 day post fertilization (dpf)); lower panel). (B) PCR analysis identified germline transmission in 8 out of 8 pools of 1 dpf embryos obtained by breeding an injected adult with a wild-type animal. PCR (+): positive PCR control, PCR (-): negative PCR control. |