FIGURE
Figure 3
- ID
- ZDB-FIG-210902-281
- Publication
- Liu et al., 2021 - Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM
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Figure 3
Chemically modified crRNA and tracrRNA duplex increased the ScCas9 efficiency. (A), Schematic illustration of the ScCas9 mdgRNP complex. The crRNA and tracrRNA scaffold with MS modified nucleotides (flag) at both termini coupled with the ScCas9 protein were loaded to the chromosome. (B), Statistics of gene editing efficiency induced by sgRNP, dgRNP and mdgRNP. ns, no significant difference; * p value < 0.05; ** p value < 0.01; *** p value < 0.001. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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