FIGURE
Figure 2
- ID
- ZDB-FIG-210902-280
- Publication
- Liu et al., 2021 - Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM
- Other Figures
- All Figure Page
- Back to All Figure Page
Figure 2
Genome editing in zebrafish using ScCas9. (A), Stem-loop differences between the ScCas9 original sgRNA tetraloop (1) and SpCas9 sgRNA tetraloop (2). (B), T7E1 assay of ScCas9/rpl17-NAG RNP-induced indel mutations. BsrBI-digested fragments of the purified PCR products were used as Ctl (+) and the undigested ones as Ctl (−). (C), Mutation frequency of ScCas9 at NNG PAM sites in zebrafish. The PAM is marked in red and underlined, and the downstream sequence of the PAM is marked in blue. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Cells