FIGURE

Figure 1

ID

ZDB-FIG-210902-279

Publication

Liu et al., 2021 - Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM

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Figure 1

ScCas9 mRNA and protein induced tyr gene editing in zebrafish. (A), Schematic illustrating the ScCas9 system used in zebrafish. The ScCas9 system consists of two components, a dual bpNLS-tagged zebrafish codon-optimized ScCas9 protein and a sgRNA comprising a 20-nt seed sequence (green) infused with a sgRNA scaffold (blue). The ScCas9-bpNLS mRNA was transcribed in vitro from Sp6 promoter and sgRNA was transcribed from T7 promoter. ScCas9 protein tagged with bpNLS and His-tag was induced expression in vitro. Red triangle indicates the double stranded break sites induced by Cas9. bpNLS, bipartite nuclear localization signal; His, histidine tag. (B), Phenotypic evaluation of embryo pigment levels induced by targeting tyr using ScCas9 according to the amount of melanin. (C), Statistics of gene editing efficiency in the tyr target site induced by SpCas9 mRNA, ScCas9 mRNA, SpCas9 protein and ScCas9 protein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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