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Figure 2
Genome editing in zebrafish using ScCas9. (A), Stem-loop differences between the ScCas9 original sgRNA tetraloop (1) and SpCas9 sgRNA tetraloop (2). (B), T7E1 assay of ScCas9/rpl17-NAG RNP-induced indel mutations. BsrBI-digested fragments of the purified PCR products were used as Ctl (+) and the undigested ones as Ctl (−). (C), Mutation frequency of ScCas9 at NNG PAM sites in zebrafish. The PAM is marked in red and underlined, and the downstream sequence of the PAM is marked in blue.
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