FIGURE

Figure 1

ID

ZDB-FIG-210902-118

Publication

Oliveira et al., 2021 - Zebrafish Model as a Screen to Prevent Cyst Inflation in Autosomal Dominant Polycystic Kidney Disease

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

PC2 staining along the KV cilia from 10 ss embryos. (A–I) Confocal images showing immunolocalization of PC2 in KV cells at the 10–11 ss in WT (A–C), mismatch-MO (D–F), and PC2-knockown (pkd2-augMO) (G–I) embryos. White arrowheads indicate PC2 detected along cilia and dashed arrows indicate PC2 at the cilia basal body. (J) Quantification of the percentage of cilia having PC2 signal along their membrane (gray bars) versus those with no PC2 staining along their membrane (white bars) in WT (n = 16), mismatch-MO (n = 8), and PC2-knockown (n = 8) embryos, immunodetected for acetylated α-tubulin. All samples followed a normal distribution and presented equal variances. Differences were, thus, statistically tested by t-test, **** p < 0.0001. Scale bars: 10 μm.

Expression Data

Antibodies:	Ab1-tuba Ab2-pkd2
Fish:	AB AB + MO9-pkd2
Knockdown Reagent:	MO9-pkd2
Anatomical Terms:	Kupffer's vesicle ciliary basal body Kupffer's vesicle ciliary membrane Kupffer's vesicle cilium
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO9-pkd2
Knockdown Reagent:	MO9-pkd2
Observed In:	Kupffer's vesicle ciliary basal body Kupffer's vesicle ciliary membrane
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.