FIGURE

Fig. 6

ID

ZDB-FIG-210825-6

Publication

Watchon et al., 2021 - Sodium valproate increases activity of the sirtuin pathway resulting in beneficial effects for spinocerebellar ataxia-3 in vivo

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Fig. 6

Treatment with sodium valproate (valproate) induces activity of the autophagy pathway in transgenic MJD zebrafish and human ataxin-3 expressing HEK293 cells. A Immunoblots of 6 dpf EGFP-Ataxin-3 84Q zebrafish treated with either valproate or vehicle control were probed with several markers of the autophagy pathway. Quantification of B beclin-1, C p62 and D LC3-II each revealed a significant increase with valproate treatment (*p = 0.014, p = 0.029 and p = 0.026 respectively, n = 3–6). E) Valproate treatment of HEK293 cells expressing Ataxin-3 84Q resulted in similar p62 levels, but increased LC3II levels. Quantification of these substrates revealed F p62 was not significantly different between valproate and vehicle treatment (p = 0.518) whilst G LC3II levels were significantly different for valproate compared to vehicle treated (p = 0.031, n = 4). Comparisons between vehicle and valproate treatment were compared using unpaired student t-tests

Expression Data

Gene:	becn1
Fish:	mq15Tg; mq17Tg
Condition:	chemical treatment by environment: valproate
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mq15Tg; mq17Tg
Condition:	chemical treatment by environment: valproate
Observed In:	whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

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