IMAGE

Fig. 6

ID

ZDB-IMAGE-210825-10

Genes

becn1

Publication

Watchon et al., 2021 - Sodium valproate increases activity of the sirtuin pathway resulting in beneficial effects for spinocerebellar ataxia-3 in vivo

All Figures

Figures for Watchon et al., 2021

Image

Figure Caption

Fig. 6

Treatment with sodium valproate (valproate) induces activity of the autophagy pathway in transgenic MJD zebrafish and human ataxin-3 expressing HEK293 cells. A Immunoblots of 6 dpf EGFP-Ataxin-3 84Q zebrafish treated with either valproate or vehicle control were probed with several markers of the autophagy pathway. Quantification of B beclin-1, C p62 and D LC3-II each revealed a significant increase with valproate treatment (*p = 0.014, p = 0.029 and p = 0.026 respectively, n = 3–6). E) Valproate treatment of HEK293 cells expressing Ataxin-3 84Q resulted in similar p62 levels, but increased LC3II levels. Quantification of these substrates revealed F p62 was not significantly different between valproate and vehicle treatment (p = 0.518) whilst G LC3II levels were significantly different for valproate compared to vehicle treated (p = 0.031, n = 4). Comparisons between vehicle and valproate treatment were compared using unpaired student t-tests

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Brain