FIGURE

Figure 7

ID

ZDB-FIG-210819-7

Publication

Ibrahim et al., 2021 - β-Cell pre-miR-21 Induces Dysfunction and Loss of Cellular Identity by Targeting Transforming Growth Factor Beta 2 (Tgfb2) and Smad Family Member 2 (Smad2) mRNAs

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Figure 7

Tg(?miR-21) mouse islets display decreases in markers of ?-cell identity. For all experiments, tamoxifen-treated Tg(?miR-21) mice were compared to tamoxifen-treated Tg(CAG-Z-miR-21) mice and tamoxifen-treated Ins1tm1(CreERT2)Thor mice. Transcript levels of markers essential for ?-cell identity were measured by RT-PCR (A). Decreased (B) MafA and (C) Pdx1 expression was quantified in nuclei of Insulin+ cells in Tg(?miR-21) islets. (D) Decreased expression of Glut2 and Pcsk1 (E) were also observed in Tg(?miR-21) islets. Levels of Aldh1a3 were increased in Tg(?miR-21) mice as compared to littermate controls (F). Decreased expression of Tgfb2 (G) and Smad2 (H) were seen in insulin+ cells in Tg(?miR-21) islets. (I) Immunofluorescence quantification is displayed as mean � SEM for littermate controls and Tg(?miR-21) mice, respectively. Immunofluorescence intensity was quantified for 3 islets per mouse for n = 3?4 mice per group; ?p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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