FIGURE

Figure 3

ID

ZDB-FIG-210819-3

Publication

Ibrahim et al., 2021 - β-Cell pre-miR-21 Induces Dysfunction and Loss of Cellular Identity by Targeting Transforming Growth Factor Beta 2 (Tgfb2) and Smad Family Member 2 (Smad2) mRNAs

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

INS1-miR-21 cells directly target the Tgfb2 pathway. (A) The qRT-PCR analysis demonstrated that transcripts for Tgfb2 and Smad2 were decreased after miR-21 induction in INS1-miR-21 cells. (B–C) Western blot analysis showed decreased Tgfb2 and Smad2 protein expression in miR-21 induced INS1-miR-21 cells. (D)Tgfb2 and Smad2 mRNAs were enriched in the miR-21 biotinylated pulldown samples normalized to cell lysate and compared to C-elegans control miR-67 biotinylated mimic pulldown. (E–F): Luciferase assays showed that activity was decreased after miR-21 induction in cells expressing wild type 3′UTR, but not in cells expressing the mutated 3′UTR for (E)Tgfb2 and (F) Smad2. n = 3–6; ∗p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol Metab