Figure 1
- ID
- ZDB-FIG-210819-1
- Publication
- Ibrahim et al., 2021 - β-Cell pre-miR-21 Induces Dysfunction and Loss of Cellular Identity by Targeting Transforming Growth Factor Beta 2 (Tgfb2) and Smad Family Member 2 (Smad2) mRNAs
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β-cell miR-21 induction results in impaired function. (A) Construct for the miR-21 overexpressing lentiviral system (LTR=long terminal repeat, Psi=packaging signal, RRE=rev response element, TRE2=tetracycline response element, Ubc=ubiquitin, HA=tag protein, rtTA3=reverse tetracycline trans-activator, IRES=internal ribosome entry site, Neo=Neomycin cassette). (B) miR-21 induction in 48 h 5 ug/ml doxycycline-treated INS1-miR-21 cells vs. INS1-scramble control cells. (C) miR-21 induction following 24 h of 5 ng/ml IL1β treatment in INS1 cells. (D) Baseline and glucose-stimulated insulin secretion were decreased in INS1-miR-21 cells as compared to INS1-scramble cells. (E) Cytoplasmic staining for the granule marker Rab37 (a marker of secretory granules) was decreased in INS1-miR-21 cells. (F) Proinsulin to insulin ratio was increased in INS1-miR-21 cells based on quantification of staining for proinsulin and insulin. Signal intensity from experimental images was normalized to signal intensity from control images, giving a relative expression for all cell staining experiments. n = 3–12 (4–5 transductions of cells); ∗p < 0.05. |