FIGURE

Figure 4

ID
ZDB-FIG-210801-99
Publication
Quadri et al., 2021 - Phosphorylation of H3-Thr3 by Haspin Is Required for Primary Cilia Regulation
Other Figures
All Figure Page
Back to All Figure Page
Figure 4

Displacement of Dido3 from the chromatin suppresses Haspin-dependent ciliary defects. Control or Dido3∆NT MEFs were driven in G0 by 48 h serum-starvation to induce ciliation and then treated overnight with DMSO or 10 nM 5-ITu ((a) and time 0 of (c)). At the end of the treatment, cells were incubated with a serum-containing medium to induce cilia resorption (in (c) for 2 and 24 h). Cilia length was measured by immunofluorescence analyzing acetylated tubulin and γ-tubulin (a). Representative images are shown in (a) (scale bar: 10 µm); cilia length is reported in graph (b); boxes include 50% of the data points, notch represent confidence interval (median ± 1.58 IQR/sqrt(n)). t-test was applied as a statistical measurement, n.s.; not significant, *** p < 0.005. Panel (c) shows cilia resorption kinetics represented as the normalized percentage of ciliated cells 2 h or 24 h after serum re-addition post-release. Error bars represent standard deviation.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.