Figure 5.

Administration of MIT-001 in mice led to a protective effect against Mabc-R infection. (a) WT BMDMs were infected with Mabc-R (MOI = 5) for 2 h in the presence or absence of MIT-001 (20 µM) and subjected to MitoSOX Red staining (Left, representative images; right, quantitative analysis). Scale bar, 50 μm. (b-e) WT mice (n = 5 each group) were left uninfected or infected intranasally with Mabc-R (1 × 10⁷ CFU), prior to treatment with or without MIT-001 (30 mg/kg) and monitored at 10 days post-infection (dpi). Lung tissues were subjected to a (b) pulmonary CFU assay, (c) qRT-PCR analysis for cytokines/chemokines, (d) TEM analysis. Mitochondria with complete cristae are shown in a; swollen mitochondria with vacuolation in the cristae are shown in b. Right, Quantitative analysis of at least 8 EM images in the lung tissues from each group of mice infected intranasally with Mabc-R (1 × 10⁷ CFU; n = 3 each group). The ratio of damaged mitochondria in total mitochondria was calculated quantitatively. Scale bars, 200 nm. (e) qRT-PCR analysis for Sirt3 mRNA expression. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant. One-way ANOVA (a right) or non-parametric test (b, c, d right and e). Data represent three independent experiments (a left and d), and values represent means (± SEM) from three or four independent experiments performed in triplicate (a right, b, c, and e).