Figure 3.

SIRT3 is essential for the amelioration of proinflammatory cytokine expression and controlling mitochondrial ROS production in BMDMs during Mabc-R infection. (a) BMDMs from SIRT3 WT and KO mice were infected with Mabc-R (MOI = 3) and incubated for 3, 6, or 18 h. (b and c) BMDMs (b) and PMs (c) were prepared from SIRT3 WT and KO mice, and were infected with Mabc-R (MOI = 3) in the presence or absence of 3-TYP (50 µM) for 3 h and quantitative real-time PCR analysis for Tnf, Il6, and Cxcl2 was performed. (d) SIRT3 WT and KO BMDMs were infected with Mabc-R (MOI = 3) for 2 h and subjected to MitoSOX Red staining (representative images are shown in the left panel; quantitative analysis is shown in the right panel). Scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001. U, uninfected. One-way ANOVA (a-d). Data are representative of three independent experiments (d left), and values represent means (± SEM) from three or four independent experiments performed in triplicate (a-c, d right).