Figure 4.

SIRT3 is required for mitochondrial OXPHOS function and attenuation of cell death during Mabc-R infection. (a and b) SIRT3 WT and KO mice (n = 8 each group) were infected intranasally with Mabc-R (1 × 10⁷ CFU) and monitored at 1 and 3 dpi. Lung tissues were collected and subjected to (a) Western blot analysis for measurement of OXPHOS protein expression (Left, representative images; right, quantitative analysis), and (b) qRT-PCR analysis. (c) Oxygen consumption rate (OCR) analysis of SIRT3 WT and KO BMDMs untreated or treated with Mabc-R for 18 h. (d) Quantitative analysis of basal respiration, spare respiratory capacity (SRC), ATP production, and maximal respiration analysis from (c). (e) PI staining after infection (Left, representative images; right, quantitative analysis). Scale bar, 300 μm. *P < 0.05, **P < 0.01, ***P < 0.001. n.s., not significant. Paired t-test (a right); non-parametric test (b and e right); One-way ANOVA (d). Data are representative of three independent experiments (a left, c, and e left), and values represent means (± SEM) from three or four independent experiments performed in triplicate (a right, b, d, and e right).