FIGURE

FIGURE 5

ID

ZDB-FIG-210718-36

Publication

Hsiao et al., 2021 - The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects

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FIGURE 5

The mRNA levels of enzymes involved in FOCM in larvae. (A) FD larvae harvested at 5-dpf were subjected to mRNA extraction and RT-PCR analysis for the expression of folate enzymes. (B) Larvae displaying FD induced by heat-shock at 1, 3, and 5 dpf were harvested at 7-dpf and examined for the expression of folate enzymes with RT-PCR. (C) FD larvae collected at the indicated stages were examined for the developmental timing-specific expression of mthfd1L during embryogenesis with real-time PCR. Ctl, control; FD, folate deficiency; aldh1l1, zebrafish 10-formyltetrahydrofolate dehydrogenase; shmt1, cytosolic serine hydroxymethyltransferase; shmt2, mitochondrial serine hydroxymethyltransferase; dhfr, dihydrofolate reductase; mthfr, methylenetetrahydrofolate reductase. ***p-value < 0.001; **p-value < 0.01; *p-value < 0.05.

Expression Data

Genes:	aldh1l1 dhfr mthfd1l mthfr shmt1 shmt2
Fish:	WT cku1Tg
Condition:	heat shock
Anatomical Term:	whole organism
Stage Range:	Prim-15 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cku1Tg
Condition:	heat shock
Observed In:	whole organism
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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