FIGURE 5

FIGURE 5

The mRNA levels of enzymes involved in FOCM in larvae. (A) FD larvae harvested at 5-dpf were subjected to mRNA extraction and RT-PCR analysis for the expression of folate enzymes. (B) Larvae displaying FD induced by heat-shock at 1, 3, and 5 dpf were harvested at 7-dpf and examined for the expression of folate enzymes with RT-PCR. (C) FD larvae collected at the indicated stages were examined for the developmental timing-specific expression of mthfd1L during embryogenesis with real-time PCR. Ctl, control; FD, folate deficiency; aldh1l1, zebrafish 10-formyltetrahydrofolate dehydrogenase; shmt1, cytosolic serine hydroxymethyltransferase; shmt2, mitochondrial serine hydroxymethyltransferase; dhfr, dihydrofolate reductase; mthfr, methylenetetrahydrofolate reductase. ***p-value < 0.001; **p-value < 0.01; *p-value < 0.05.