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Hsiao et al., 2021 - The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects

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FIGURE 5

The mRNA levels of enzymes involved in FOCM in larvae. (A) FD larvae harvested at 5-dpf were subjected to mRNA extraction and RT-PCR analysis for the expression of folate enzymes. (B) Larvae displaying FD induced by heat-shock at 1, 3, and 5 dpf were harvested at 7-dpf and examined for the expression of folate enzymes with RT-PCR. (C) FD larvae collected at the indicated stages were examined for the developmental timing-specific expression of mthfd1L during embryogenesis with real-time PCR. Ctl, control; FD, folate deficiency; aldh1l1, zebrafish 10-formyltetrahydrofolate dehydrogenase; shmt1, cytosolic serine hydroxymethyltransferase; shmt2, mitochondrial serine hydroxymethyltransferase; dhfr, dihydrofolate reductase; mthfr, methylenetetrahydrofolate reductase. ***p-value < 0.001; **p-value < 0.01; *p-value < 0.05.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol